Clinical features of the recurrence of idiopathic benign paroxysmal positional vertigo / 临床耳鼻咽喉头颈外科杂志

To investigate the clinical features of the recurrence of idiopathic benign paroxysmal positional vertigo(IBPPV)patients.Patients with IBPPV were enrolled and were followed-up for 36 months after being well controlled.The data of the patients including age,gender,and co-morbidities(hypertension,diabetes,hyperlipidemia)were analyzed.Characteristics of the patients with recurrent BPPV were compared with those without recurrence.Two hundred and one patients were enrolled and twenty-two(10.9%)patients presented recurrent IBPPV within 36 months.Among them,about 16% showed changes in the involved semicircular canals.50% recurrence occurred within 6 months after the first treatment.The recurrence rate of BPPV in 50-60 years old patients(50%)is higher than other patients(P=0.04).No significant difference in terms of gender or co-morbidities(hypertension,diabetes,hyperlipidemia)was observed between the two groups.The incidence of recurrence in idiopathic BPPV patients was 10.9%in the present study.The mean period of 50% recurrence after a symptom-free interval was about 6 months.Furthermore,different semicircular canals were involved in about 50% of patients during recurrence.BPPV recurrence was not correlated with age,gender or co-morbiditie.

Dissection of inner ear neuroepithelium in zebrafish / 中华耳鼻咽喉头颈外科杂志

<p><b>OBJECTIVE</b>To establish a model by using zebrafish for the inner ear development and disease research, to explore the possibility of complete dissection of inner ear neuroepithelium in zebrafish by surface preparation of inner ear maculae.</p><p><b>METHODS</b>The inner ear samples of zebra fish were fixed followed by harvesting. After decalcified and dehydrated, the coronal cryosections were made at 3 µm thickness at -23°C using cryostat, the hematoxylin and eosin staining and immunofluorescence and histochemistry staining were performed, the saccules, utricles and lagenaes were successfully obtained by fine dissection. The sensory maculae were mounted on glass and then were stained.</p><p><b>RESULTS</b>After hematoxylin and eosin staining and immunofluorescence and histochemistry staining of cryosections, the sensory maculae of inner ear could be determined by otoliths. On the basis of distinguish of otoliths, the sensory maculae of inner ear could be finely dissected, the overall integrity of the sensory maculae could be preserved completely. After immunofluorescence and histochemistry staining, intact epithelia with strong hair cell bundle staining could be seen.</p><p><b>CONCLUSIONS</b>The neuroepithelium hair cell examination of zebra fish can be entirely attained by surface preparation of inner ear maculae.</p>

Neuronal degeneration of the auditory nervous center in the mouse model of cochlear ablation / 中华耳鼻咽喉头颈外科杂志

<p><b>OBJECTIVE</b>To study the long-term effect of cochlear ablation on cochlear nucleus (CN).</p><p><b>METHODS</b>A total of 40 BALB/c adult mice were used in the present study. Mice were divided into experimental group and control group at random. In experimental group, the cochlea was destroyed with stainless steel needle under the anatomy microscopy. Mice were tested for ABR thresholds at second day and 4 months after operation to assess hearing sensitivity. Animals were allowed to survive for four months. Histological sections of the cochlear nucleus were evaluated with serial sections stained with Nissl staining and silver staining alternatively. Morphological change in anteroventral cochlear nucleus (AVCN), posteroventral cochlear nucleus (PVCN), dorsal cochlear nucleus (DCN) and octopus cell area (OCA) was observed evaluated four month later after the cochlear ablation.</p><p><b>RESULTS</b>In the experimental group, after operation, early components of ABR couldn't be recognized. The volume of CN was decreased dramatically and the cells were less comparing to the control group. The volume of AVCN after cochlear ablation showed a decline to 22% less than the control group, and the number of neurons also dropped by 25%. In PVCN, the decreased volume by 40% with significant neuronal loss of 47% was observed four month later after the cochlear ablation. And in DCN, the volume shrinking to 24% and the loss of neurons reached to 39%. The most significant change was observed in OCA (octopus cell area), with the neuron numbers, the area and the volume of OCA decreased by 60%, 19%, and 47% respectively. There was a statistically significant difference (P<0.05, Mann-Whitney U test) in the morphological changes between the two groups.</p><p><b>CONCLUSION</b>Neuronal degeneration was observed in cochlear nucleus after four months cochlear ablation.</p>